Description
ROTI®Prep gDNA Mini 2.0, 10 pieces, pour la biologie moléculaire, Density (D) ~1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep gDNA MINI 2.0 was developed to isolate genomic DNA from a wide variety of starting materials.The isolation is based on a new procedure and combines sample lysis with the subsequent binding of the DNA to the surface of a column membrane. After several washing and centrifugation steps, the DNA is eluted from the membrane using an elution buffer. The extraction procedure in this kit is optimised to achieve maximum yield.
- For DNA extraction from bacteria, fungi and eukaryotic cell, blood and tissue samples.
- Preparation in the well-established mini-column system
- Fast, simple and reliable
- Extraction time of approx. 8 min (without lysis step)
- Yield, depending on the starting material, of up to 65 µg gDNA
- Microbial cell cultures (max. 5x1010)
- Fungi and eukaryotic cell cultures (max. 5x106)
- Rodent tail (0.5-1 cm)
- Oral mucosal swabs
- Tissue samples
- Blood (200-400 µl)
Lysis Buffer LSV, Lysis Buffer LSN, Binding Buffer BR, Binding Buffer BSN, Proteinase K, RNase A (10 mg/ml), Washing Buffer WSO, Washing Buffer WSH (conc.), Washing Buffer WST (conc.), Elution Buffer EB, Centrifugation columns, Collection tubes, Elution tubes, detailed instructions.Ethanol 99.8 % (p.a., Art. No. 9065), RNase free water (Art. No. 1HPE.1), TE buffer (order no. 1052.1) and PBS (Art. No. 1058.1) are not included in the kit and should be ordered separately if required.
| Basic material | Bacteria, plants, fungi, cell cultures, animal tissue, or blood |
| Quantity of material | Microbial cell cultures (<5x1010), eukaryotic cell cultures (<5x106), rodent tail (0.5-1 cm)buccal swabs, tissue samples (<20mg)Blood (200–400 µl) |
| Preparation time | approx. 8 minutes (excluding lysis) |
| Instructions for use, addition | For DNA extraction from harder or more complex samples (bacteria, fungi) we recommend preparing the sample with a suitable ROTI®SampleLyse tube (e.g. Art.No. 1YKE.1). For DNA extraction from plant tissue, we recommend ROTI®Prep Plant DNA (Art. No. 20H4.1). |
| Version | Mini prep |
| Automatic use | no |
| Elution volume | 50-400 µl |
| Typical yield | <65 µg |
| Typical purity (A260/A280) | 1.8-2.0 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)