Description
ROTI®Transform, for prep. of competent, E. coli, sterile, ready-to-use, Density (D) 1 g/cm³, Storage temp. -20 °C
ROTI®Transform is a simple, effective and fast method of producing competent E. coli in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations.
- Flexible selection of strains
- Effective, simple and fast
- Adequate for 500 transformations
Transformation efficiency is 5 x 105 to 1 x 106 colonies per µg DNA.
Protocol:1. Resuspend cells in fresh, cold LB-medium2. Add ROTI®Transform 13. Add ROTI®Transform 2 and incubate for 5 minutes on ice.
ROTI®Transform consists of 45 ml ROTI®Transform 1 (Art. No. P041) and 5 ml ROTI®Transform 2 (Art. No. P042).
Long-term Storage of the Kit at -20 °C.Storage for use:Reagent 1: Short-term storage may be done at 4 °C. For long-term storage of over 14 days we recommend a storage temperature of -20 °C.Reagent 2: Storage at 4 °C.