ROTIPHORESE® Gel B, 2 % bisacrylamide stock solution, 250 ml, glass

Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE^® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis.

ROTIPHORESE^® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers. The ROTIPHORESE^® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas. In order to ensure a high consistency of our ROTIPHORESE^® solutions, each batch is subjected to an electrophoretic test.

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C). With the ROTIPHORESE^® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).

Acrylamide and Bisacrylamide Stock Solutions

PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C).With the ROTIPHORESE^® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B).For detailed instructions regarding gel preparation with ROTIPHORESE^® Gel A, A-40 and B please see user manual and technical information brochure “PAGE Instructions”.

Ready-to-use, stabilised 2 % bisacrylamide stock solution. In combination with ROTIPHORESE^®Gel A (30 % acrylamide stock solution, Art. No. 3037) or ROTIPHORESE^®Gel A-40 (40 % acrylamide stock solution, Art. No. 7748) gels with up to 25 % total acrylamide concentration can be produced.

For detailed instructions regarding gel preparation with ROTIPHORESE^®Gel A, A-40 and B please see our Technical Information Brochure “PAGE Instructions”.