Beschrijving
anti-Cardiac Actin muis, monoklonaal, AC1-20.4.2, 50 µg/ml, affiniteitsgezuiverd (geproduceerd door PROGEN), dichtheid (D) ~1 g/cm³, kookpunt (bp) ~100 °C, bewaartemp. +4 °C
Dit antilichaamportfolio bevat een breed scala aan weefsel- & tumorspecifieke primaire antilichamen voor fundamenteel onderzoek, productontwikkeling en analyse van uiteenlopende biologische vraagstukken. De monoklonale antilichamen zijn uitstekend geschikt voor alle cel- of weefselbiologische studies en voor de analyse van predictieve markers in onderzoek.
- Hoge epitoop-affiniteit
- Zeer specifieke detectie van het doeleiwit
- Niet-geconjugeerd
- Geschikt voor bijv. western blot, immunoprecipitatie, ELISA & immunofluorescentie
Ac1 is een uitstekende marker voor hartweefsel; het onderscheidt foetaal (cardiaal) a-actine van alle andere actine-isovormen. Foetaal actine kan na letsel gelokaliseerd worden in regenererend skeletspierweefsel (in satellietcellen) en in aderen van de navelstreng. Mab Ac1-20.4.2 vertoont geen kruisreactiviteit met andere actine-isovormen die aanwezig zijn in skelet- en gladde spieren, mits er stringente experimentele condities zijn toegepast. Reagerend polypeptide: specifiek voor de foetale (cardiale) isovorm van actine.
Immunohistochemie (IHC) - bevroren: 1:10 (5 μg/ml; voeg 0,5 M NaCl toe aan alle wasbuffers om de specificiteit te verhogen) Immunohistochemie (IHC) - paraffine: 1:10 (5 μg/ml; microgolfbehandeling aanbevolen) Western Blot (WB): 1:1.000 (50 ng/ml; voeg 1 M NaCl toe aan alle wasbuffers om de specificiteit te verhogen)
| Toepassing | IHC WB |
| Gastheersoort | muis |
| Klonale status | monoklonaal |
| Reactiviteit antilichaam | rund kip humaan muis konijn |
| Zuivering | Affiniteitschromatografie |
| Conjugaat | niet-geconjugeerd |
| Concentratie | 50 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | P68034 (kip), P68032 (humaan), G1STB6 (konijn) |
| Kloon | AC1-20.4.2 |
| Target | cardiaal a-actine |
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Questions about anti-Cardiale actine muis, monoklonaal, AC1-20.4.2, 0,2 ml, kunststof
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anti-Cardiale actine muis, monoklonaal, AC1-20.4.2, 0,2 ml, kunststof is the article on this page. Luminix Health distributes PROGEN for laboratory use. It sits in Primary antibodies, inside Antibodies & ELISA kits, in Microbiologische media.
A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.
An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.
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