Beschrijving
BICINE is een biologische PUFFERAN®-buffer, geleverd met een zuiverheid van min. 99 % voor moleculaire biologie en verwante buffertoepassingen. De verbinding heeft de empirische formule C₆H₁₃NO₄, een molaire massa van 163.2 g/mol en een dichtheid van 1.37 g/cm³ (CAS-nummer 150-25-4; EG nr. 205-755-1). Het wordt vaak gekozen als zwitterionische buffercomponent van het Good’s-type in elektroforese, enzymassays en andere moleculair-biologische buffersystemen. Dit aanbod van fabrikant Carl ROTH past BICINE in reagentenportfolio’s gericht op moleculair-biologische buffers waar gedefinieerde zuiverheid en identiteit van belang zijn. Onderzoekers kunnen de vaste stof oplossen en titreren tot de vereiste pH voor specifieke protocollen. Gedocumenteerde molaire massa en dichtheid ondersteunen een nauwkeurige bereiding van oplossingen en laboratoriumregistraties. De specificatie min. 99 % helpt de reproduceerbaarheid te behouden over herhaalde bufferbatches in onderzoeksomgevingen.
Dit artikel maakt deel uit van Biologische buffers (Tris, HEPES, MOPS), in Buffers & pH-oplossingen.
Kenmerken
- Uiterlijk: wit, krist.poeder
- Gehalte (titratie): ≥99 %
- Droogverlies: ≤1 %
- pH-waarde (1 M in H2O): 4-6
- 280 nm: ≤0.05
- 260 nm: ≤0.05
- CAS-nummer: 150-25-4
- Verpakkingsgroottes: 50 g, 250 g, 500 g
- EG-nr.: 205-755-1
- Brutoformule: C₆H₁₃NO₄
- Molaire massa: 163.2 g/mol
- Technisch datablad:
- Veiligheidsinformatieblad:
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Questions about BICINE, PUFFERAN® , min. 99 %, 50 g, kunststof
What is this article?
BICINE, PUFFERAN® , min. 99 %, 50 g, kunststof is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biologische buffers (Tris, HEPES, MOPS), inside Buffers & pH-oplossingen, in Chemicaliën.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH-oplossingen. The sub-category is Biologische buffers (Tris, HEPES, MOPS). The catalogue is Chemicaliën.
Shelves beside this one are pH-buffers & geleidbaarheidsstandaarden and Buffers (overig).
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