Beschrijving
Europese Farmacopee 2.6.7 Mycoplasma's vereist validatie van de gevoeligheid en robuustheid ten opzichte van de monstermatrix. Bovendien moet de methode een prestatie laten zien die gelijk is aan of beter is dan die van de klassieke kweekprocedure. Omdat de meeste test- en celkweeklaboratoria bang zijn om mycoplasma te kweken, is een dergelijke vergelijking niet eenvoudig te realiseren. Microsart® Validatiestandaarden zijn niet infectieus en daarom veilig in gebruik. Ze worden getitreerd tot 10 CFU/ml, de gevoeligheidslimiet voor NAT-gebaseerde methoden zoals PCR ter vervanging van de traditionele kweekmethode. De mycoplasmacellen die worden gebruikt voor de productie van de Microsart® Validatiestandaard zijn referentiestammen met een lage passage, gekweekt in kweekbouillon zoals beschreven in EP 2.6.7. De culturen worden geoogst in de vroege logaritmische fase van de groei om een atypisch hoog percentage dode mycoplasmadeeltjes te voorkomen, direct getitreerd in kweekbouillon en uitgeplaat op Hayflick- en Frey-medium voor kwantificering op basis van kolonievormende eenheden (CFU).
Recent bekeken
Misschien vind je dit ook leuk
Browse related categories
Category
Sub-category
Ready-to-use culture media (plates, tubes, bottles)
Catalogue
Brand
Questions about Microsart Validatie Standaard Achole, Microsart® Validatie Standaard Acholeplasm
What is this article?
Microsart Validatie Standaard Achole, Microsart® Validatie Standaard Acholeplasm is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Ready-to-use culture media (plates, tubes, bottles), inside Culture media & microbiology, in Media.
A ready-to-use plate, tube or bottle is the medium already prepared and sterile. It saves the autoclave and it expires. The fill volume, the supplements and the storage temperature are the specification. A plate that has dried out or grown a colony of its own is finished.
A culture medium is the food and the solid or liquid home of a microorganism. Dehydrated media are powders the laboratory dissolves and sterilises. Ready-to-use plates and tubes are already that. A Petri dish is the plate. Selective media let one kind of organism grow and hold another back. The formulation and the supplements, not the colour of the agar, are the specification. Reference strains are at ATCC. Methods are on PubMed. Biosafety practice is the WHO laboratory biosafety topic.
Which category should I open next?
The category is Culture media & microbiology. The sub-category is Ready-to-use culture media (plates, tubes, bottles). The catalogue is Media.
Shelves beside this one are Dehydrated culture media (agar & broth bases), Petri dishes, Microbiology (other), Inoculation loops, spreaders & needles and Microbial identification, hygiene monitoring & rapid tests.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.