Beschrijving
F-12 (HAM) Nutrient Mixture, 500 ml is a cell culture medium originally designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. The formulation is modified with L-glutamine, glucose, phenol red, sodium pyruvate and sodium bicarbonate to support demanding mammalian culture conditions. Supplied as a 500 ml unit with a net weight of 0,500 kg, this nutrient mixture from manufacturer Sartorius offers a ready-to-use basis for CHO plating and related cell culture work in research and development laboratories. Phenol red allows convenient visual pH assessment, while L-glutamine, glucose and sodium pyruvate contribute essential carbon and energy sources for cell maintenance. Sodium bicarbonate supports buffering under standard incubator atmospheres. The 500 ml pack size suits routine medium preparation and change schedules. This Ham’s F-12 mixture integrates smoothly into serum-free CHO protocols and other mammalian workflows that require a balanced nutrient composition with clearly defined additives.
This article is part of Cell culture media & sera, in Cell culture.
Key features
- Pack size: 1 pc
- Storage temperature: 2–8 °C
- Product type: Ham's F-12 nutrient mixture
- Volume: 500 ml
- Additives: L-glutamine, glucose, phenol red, sodium pyruvate, sodium bicarbonate
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Questions about Nutrient Mixture F-12 (Ham)
What is this article?
Nutrient Mixture F-12 (Ham) is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Cell culture media & sera, inside Cell culture, in Media.
Cell-culture medium is the liquid a cell line grows in. Serum, often fetal bovine, supplies what the basal medium does not. With or without glutamine, with or without phenol red, and the serum origin are different products. Serum is a cold-chain article and is never part of free standard delivery.
Cell culture grows cells outside the body. The medium, often with serum, supplies what the flask does not. A flask or a dish is the vessel, treated so the cells attach or left untreated so they float. Contamination by microbes, or by another cell line, is the ordinary failure. Cold-chain articles in this work, including serum, are never part of free standard delivery. Lines are catalogued by ATCC. Papers are on PubMed. Practice is summarised by the WHO laboratory biosafety topic.
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