Beschrijving
Proteinase K, gevriesdroogd, is een breedspectrum serineprotease met een minimale activiteit van 30 U/mg voor workflows in de moleculaire biologie. Geïdentificeerd door CAS-nummer 39450-01-6 en EG-nr. 254-457-8, wordt dit enzym op grote schaal toegepast in DNA- en RNA-isolatieprotocollen waarbij een efficiënte vertering van eiwitten en inactivatie van nucleasen vereist zijn. De gevriesdroogde vorm maakt een gemakkelijke opslag en reconstitutie vlak voor gebruik mogelijk. Aangeboden door fabrikant Carl ROTH, kan Proteinase K eenvoudig worden geïntegreerd in bestaande isolatiekits en aangepaste buffersystemen. De robuuste proteolytische activiteit onder denaturerende condities maakt het een standaardkeuze voor het opzuiveren van eiwitrijke lysaten voorafgaand aan stroomafwaartse amplificatie-, sequentie- of hybridisatie-assays.
Dit artikel behoort tot Enzymen & substraten, in Biochemicaliën & reagentia.
Belangrijkste kenmerken
- Uiterlijk: wit poeder
- Specifieke activiteit: ≥30 U/mg
- DNasen, RNasen: niet gedetecteerd
- CAS-nummer: 39450-01-6
- Verpakkingsgrootten: 100 mg, 1 g, 5 g, 500 mg, 25 mg
- EG-nr.: 254-457-8
- Opslagtemperatuur: -20 °C
- Vorm: Gevriesdroogd
- Technisch informatieblad:
- Veiligheidsinformatieblad:
Compliance & Safety Information
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Questions about Proteinase K, lyof., min. 30 E/mg, 25 mg, glas
What is this article?
Proteinase K, lyof., min. 30 E/mg, 25 mg, glas is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Media.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
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The category is Biochemicals & reagents. The sub-category is Enzymes & substrates. The catalogue is Media.
Shelves beside this one are Amino acids & peptides, Carbohydrates & sugars, Coenzymes, nucleotides & vitamins, Biochemicals (other), Antibiotics & antimycotics, Lipids, steroids & fatty acids and Detergents & surfactants.
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