Beschrijving
ROTI®Cell Trypsine/EDTA-oplossing (10x), steriel, 10x, CELLPURE®, Dichtheid (D) 1 g/cm³, Opslagtemp. -20 °C
Trypsine is een eiwitsplitsend spijsverteringsenzym uit de alvleesklier dat behoort tot de serineproteasen en dat lysine en arginine aan de C-terminus splitst. Trypsine-oplossingen worden gebruikt voor het scheiden van cellen van weefsel of celmonolagen. Ze zijn daarom essentieel voor routinematige toepassingen bij het passeren van celculturen, voor celkweekexperimenten om een adequaat aantal cellen te verkrijgen of voor de dissociatie van primair weefsel. Als alternatief voor trypsine kan de celvriendelijkere oplossing ROTI®Cell Accutase worden gebruikt, die vrij is van dierlijke componenten.
Deze trypsine-oplossing bevat EDTA (0,22%). EDTA is een chelaatvormer en verbetert het vermogen van trypsine om adherente cellen los te maken. EDTA bindt calcium en magnesium, wat de celcontacten verder verzwakt. Dit bevordert de hydrolyse van specifieke peptidebindingen door trypsine.
De benodigde trypsineconcentratie hangt voornamelijk af van het celtype en de leeftijd van de celcultuur. Ter oriëntatie vindt u een volledig protocol in de technische informatiebrochure.
Na celdissociatie moet trypsine worden geïnhibeerd. Dit kan door middel van FCS of trypsine-inhibitoren.
| Eindconcentratie | 1x |
| Enzym | Trypsine uit varkensalvleesklier |
| Alternatieve naam | Celdissociatiereagentia |
| Gebruik | Celdissociatie |
| Gebruiksaanwijzing, toevoeging | Bereiding van aliquots aanbevolen om herhaald invriezen en ontdooien te vermijden. |
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Questions about ROTI®Cell Trypsine/EDTA-oplossing (10x), steriel, 10x, CELLPURE®, 100 ml, kunststof
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ROTI®Cell Trypsine/EDTA-oplossing (10x), steriel, 10x, CELLPURE®, 100 ml, kunststof is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Microbiologische media.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
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