Description
TBE Buffer (5X) is a solution used in Agarose Gel Electrophoresis (AGE) typically for the separation of nucleic acids (i.e. DNA and RNA). Tris-Borate-EDTA (TBE) is not only used in nucleic acid agarose and polyacrylamide gel electrophoresis but also in agarose and polyacrylamide gel preparation.Instructions for use:1. Take a bottle of TBE Electrophoresis Buffer (5X) from specified storage conditions at 15-30°C and read the label.2. Ensure that the cap of the bottle is tight.3. Gently swirl the solution in the bottle to ensure homogeneity.4. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.5. Using aseptic/sterile technique under a laminar-flow culture hood and work according to established protocols.6. The TBE Electrophoresis Buffer Concentrate should be diluted to a working concentration of 1X before use. 7. For each Electrophoresis, use fresh TBE Buffer at the working concentration of 1X.