Description
Geneticin disulphate (G418 Sulphate), min. 650 U/mg, for biochemistry, Molar mass (M) 692,70 g/mol, Storage temp. +4 °C, CAS No. 108321-42-2, Empirical formula C20H40N4O10 · 2 H2SO4
Inhibits protein biosynthesis by binding to ribosomes, particularly to eukaryotic 80S ribosomes. Through interference with the tRNA docking process, sequence errors appear and the elongating peptide chain is truncated. Geneticin disulphate unspecifically effects all cells, and is highly toxic for eukaryotic cells. Resistance is mediated by the bacterial enzyme aminoglycoside-3-phosphotransferase.
Application: Selection of transfected eukaryotic cells. Resistance gene: Neomycin.