Description
Ponceau S (C.I. 27195) is a sulfonated azo dye supplied for histology and electrophoresis, with CAS number 6226-79-5 and EG number 228-319-2. The empirical formula is C₂₂H₁₂N₄Na₄O₁₃S₄ and the molar mass is 760,56 g/mol. Laboratories value Ponceau S for quick visualisation of transfer efficiency before downstream immunodetection, as well as for general protein staining workflows. The defined Colour Index designation C.I. 27195 and full chemical identifiers support reproducible preparation of working solutions and clear inventory records. provides a convenient quantity for routine electrophoresis and histology benches without excessive bulk. Physical and identity data given for the dye aid formulation of staining baths at the concentrations required by standard protocols in molecular biology and pathology laboratories.
This article is part of Microscopy stains & staining solutions, in Dyes, stains & indicators.
Key features
- Absorption max. (in H2O): 517-523 nm
- Specific extinction E 1 %/1 cm: >460
- Loss on drying (110 °C): ≤10 %
- CAS number: 6226-79-5
- Pack sizes: 25 g, 10 g
- EG nr.: 228-319-2
- Empirical formula: C₂₂H₁₂N₄Na₄O₁₃S₄
- Molar mass: 760,56 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
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Precautionary Statements (P-Phrases)
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Questions about Ponceau S (C.I. 27195), for histology and electrophoresis, 10 g, glass
What is this article?
Ponceau S (C.I. 27195), for histology and electrophoresis, 10 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Microscopy stains & staining solutions, inside Dyes, stains & indicators, in Lab chemicals.
A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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