Description
ROTI®Blue, 5x concentrated, staining solution for protein gels, Density (D) 1,24 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 1805
ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.
- Most simple application
- No destaining necessary
- 5x more sensitive than Coomassie® R 250
ROTI®Blue is approximately 5 x more sensitive than standard Coomassie® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity.Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining.Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.
| Sensitivity | 10 |
| Use | Coomassie® stainingsolution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |