Description
ROTI®Load DNA shortrun 1x, (with glycerol) 1x DNA gel load. buffer, ready-to-use, DNase-free, Density (D) 1 g/cm³, Storage temp. -20 °C
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.
ROTI®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.
ROTI®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI®Load DNA. ROTI®Load DNA gel loading buffer is usually used as 1x concentrated solution.
In order to prevent important DNA bands being clouded, ROTI®Load DNA short-run 1x (with glycerol) contains only bromophenol blue in slightly reduced concentration (see lane 2 in the product image). The buffer may also be diluted to 0,6x if the bromophenol blue in the gel could mask an important DNA band.
Glycerin is the standard reagent for increasing sample density.
Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
| Dye | Bromphenol blue |
| Incl. DNA staining | no |
| Use | Short separation distances, fast gels, pelleted DNA |
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Questions about ROTI®Load DNA shortrun 1x, (with glycerol) 1x DNA gel load. buffer, 5 ml
What is this article?
ROTI®Load DNA shortrun 1x, (with glycerol) 1x DNA gel load. buffer, 5 ml is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Electrophoresis systems & power supplies, inside Electrophoresis, in Life Science & Microbiology.
An electrophoresis system is the tank and the electrodes. A power supply holds voltage, current or power. The gel size the tank accepts and the voltage range of the supply are the specification. A power supply that cannot hold a constant voltage will not run a method that demands one.
Electrophoresis pulls charged molecules through a gel. Smaller ones move farther. Agarose gels separate nucleic acids. Polyacrylamide gels separate proteins. A comb forms the wells. A power supply holds the voltage or the current. The gel percentage is the sieve. A marker lane is how the bands are named. The methods are in the PubMed literature. Sequences of the nucleic acids are on NCBI Nucleotide.
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The category is Electrophoresis. The sub-category is Electrophoresis systems & power supplies. The catalogue is Life Science & Microbiology.
Shelves beside this one are Gels, combs & casting accessories.
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