Description
ROTI® Phenol/Chloroform/Isoamyl alcohol is a ready-to-use extraction mixture formulated for isolation of nucleic acids in molecular biology workflows. The liquid reagent is intended for DNA and RNA purification steps in which phase separation cleanly removes proteins and other contaminants from aqueous nucleic acid samples. Physical data include a density of 1,2 to 1,3 g/cm³, a flash point above 80 °C, and a boiling point above 61 °C, supporting safe handling planning and process documentation. This preparation from manufacturer Carl ROTH is positioned for DNA/RNA isolation and related extraction systems where a consistent phenol–chloroform–isoamyl alcohol blend is required. Ready-to-use format reduces on-bench mixing variability and helps standardise organic extraction steps across molecular biology and life-science applications.
This article belongs on DNA & RNA extraction kits, in Molecular biology.
Key features
- Pack sizes: 250 ml, 500 ml, 100 ml
- Density: 1,2 to 1,3 g/cm³
- Flash point: >80 °C
- Boiling point: >61 °C
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
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Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Phenol/Chloroform/Isoamyl alcohol, for extraction of nucleic acids, 100 ml
What is this article?
ROTI®Phenol/Chloroform/Isoamyl alcohol, for extraction of nucleic acids, 100 ml is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in DNA & RNA extraction kits, inside Molecular biology, in Life Science & Microbiology.
An extraction kit lyses the sample and then binds the nucleic acid, usually on a column or on a bead, and washes the rest away. Kits for blood, tissue, soil or a PCR cleanup are different chemistries. Yield and the size of fragments they keep are in the protocol. An RNA kit has to protect against RNase.
Molecular biology here is the handling of DNA and RNA. Extraction kits break the cell and then separate the nucleic acid from protein and from the other nucleic acid. PCR and qPCR reagents copy a chosen sequence so it can be seen. The enzymes and the nucleotides are what the copy depends on. Nuclease contamination destroys the sample before the kit can save it. Sequences are on NCBI Nucleotide. Papers are on PubMed.
Which category should I open next?
The category is Molecular biology. The sub-category is DNA & RNA extraction kits. The catalogue is Life Science & Microbiology.
Shelves beside this one are Molecular biology reagents (other) and PCR, qPCR & nucleic acid analysis reagents.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.