Description
ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, for DNA sequencing, Density (D) 1 g/cm³, Storage temp. +4 to +15 °C
- For preparation of 4-20 % sequencing gels
- Most simple handling of three optimised ready-to-use solutions
- No tedious dissolving of urea
- Prevents acrylamide dusts
- Reproducible, superior quality of gels
- In tried-and-tested ROTIPHORESE® quality
Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®Sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %.
Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).
- 1 l ROTIPHORESE®sequencing gel concentrate (Art. No. 3043.1) - ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1 with 50 % urea (8,3 M).
- 1 l ROTIPHORESE®sequencing gel diluent (Art. No. 3047.1) - 50 % (g/v; 8,3 M) solution of urea in water.
- 250 ml ROTIPHORESE®sequencing gel buffer concentrate (Art. No. 3050.1) - 10x TBE with 50 % urea (8,3 M).
- Detailed instructions for use are enclosed with the product.
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)