Description
ROTIPHORESE® Sequencing gel diluent is a ready-to-use aqueous solution containing 50 % urea (w/v, 8,3 M) for the preparation of sequencing and PAGE gels. With a density of 1,12 g/cm³ and a boiling point of 100 °C, the diluent provides a convenient urea stock for vertical electrophoresis workflows. It is formulated in water and supplied ready for direct use in gel-casting protocols that require high urea concentrations. Available in a 1 l glass pack from manufacturer Carl ROTH, the product supports laboratories performing DNA sequencing gels and denaturing polyacrylamide electrophoresis. Consistent urea content helps maintain the denaturing conditions essential for high-resolution nucleic-acid separations. Molecular biology and biochemistry laboratories can incorporate this diluent into established ROTIPHORESE® and PAGE gel-preparation procedures. The litre pack size suits both occasional and higher-throughput gel casting. Overall, the formulation simplifies preparation of urea-containing sequencing gels while matching the physical properties expected for aqueous urea diluents in electrophoresis.

