Description
RPMI 1640 Medium without L-glutamine and phenol red is a basal cell-culture medium developed for the long-term culture of blood cells and human leukocytes, including neoplastic white blood cells. Supplied in a 500 ml pack, this modification contains glucose and sodium bicarbonate while omitting both L-glutamine and phenol red. Laboratories therefore retain full control over glutamine addition and benefit from optical clarity for photometric or fluorescence-based readouts. Originally optimised for leukocyte work, the formulation is now widely employed as a general serum-supplemented medium for hybridoma cultures. Its balanced nutrient and salt profile supports growth of a broad range of mammalian suspension and adherent cells under standard incubator conditions. Offered by manufacturer Sartorius, the medium fits routine research and production workflows. Absence of the pH indicator dye makes it especially suitable when colourimetric assays or live-cell imaging must remain free from background interference. Users simply supplement with L-glutamine or a stable glutamine analogue according to protocol, ensuring flexibility across diverse cell lines and experimental designs in cell biology and hybridoma applications.
This article is part of Click chemistry, bioconjugation & synthesis reagents, in Biochemicals & reagents.
Key features
- Pack size: 500 ml
- Storage temperature: 2–8 °C
- Formulation: With glucose and sodium bicarbonate
- Without: L-glutamine and phenol red
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Questions about RPMI Medium 1640 w/o Phenol Red 500ML
What is this article?
RPMI Medium 1640 w/o Phenol Red 500ML is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Click chemistry, bioconjugation & synthesis reagents, inside Biochemicals & reagents, in Microbiology lab supplies.
Click and bioconjugation reagents join two molecules under mild conditions, often an azide to an alkyne, or an NHS ester to an amine. The linker length and the reactive group are the specification. They are small bottles of a reactive compound, and moisture often ruins them.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
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