Description
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt is a chromogenic phosphatase substrate identified by CAS number 6769-80-8 and the empirical formula C₈H₅BrClNO₄P·C₇H₁₀N. With a molar mass of 433,63 g/mol, the compound is Cleavage of the phosphate ester liberates a halogenated indoxyl intermediate that oxidises to a coloured indigo dye, allowing straightforward detection of phosphatase activity by eye or photometry. This reagent from manufacturer Carl ROTH is used in enzyme assays, blotting techniques and detection protocols that depend on indolyl phosphate chemistry. The p-toluidine salt form and documented molecular parameters support accurate weighing and solution preparation in research laboratories. Scientists apply the substrate when screening alkaline phosphatase, optimising chromogenic methods or performing related life-science analyses. size provides a practical balance of handling convenience and working quantity for repeated experimental use.
This article belongs on Microscopy stains & staining solutions, in Dyes, stains & indicators.
Key features
- CAS number: 6769-80-8
- Pack sizes: 2 g, 5 g, 10 g, 25 g
- Empirical formula: C₈H₅BrClNO₄P·C₇H₁₀N
- Molar mass: 433,63 g/mol
- Storage temperature: -20 °C
- Product category: Biochemicals
- Technical Information Sheet:
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Questions about 5-Bromo-6-chloro-3-indolyl phosphate p-t, oluidine salt, 2 g
What is this article?
5-Bromo-6-chloro-3-indolyl phosphate p-t, oluidine salt, 2 g is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Microscopy stains & staining solutions, inside Dyes, stains & indicators, in Lab chemicals.
A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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