Description
5-Carboxytetramethylrhodamine succinimidyl ester is an amine-reactive fluorescent dye with CAS number 150810-68-7 and the empirical formula C₂₉H₂₅N₃O₇. Its molar mass is 527,52 g/mol. This N-hydroxysuccinimide ester of the 5-carboxy isomer of tetramethylrhodamine enables covalent attachment to primary amino groups on proteins, peptides, antibodies and other biomolecules. size is convenient for laboratory-scale bioconjugation, probe synthesis and fluorescence-based detection methods including microscopy, blotting and chromatographic analysis. Distinct isomeric purity relative to the 6-carboxy analogue can be important when spectral or chromatographic behaviour must be tightly controlled. Available from manufacturer Carl ROTH, the reagent supports reproducible labelling protocols in academic and industrial life-science environments. Standard NHS-ester coupling conditions in slightly alkaline aqueous or mixed organic media afford efficient dye incorporation. The defined molecular formula and CAS identity facilitate documentation in validated analytical and research procedures.
This article is part of Fluorescent dyes & labels, in Dyes, stains & indicators.
Key features
- Purity (HPLC): ≥ 95 %
- Colour: red to purple
- Form: powder to crystalline powder
- ¹H-NMR Spectrum: corresponds to assigned structure
- CAS number: 150810-68-7
- Pack sizes: 50 mg, 100 mg, 250 mg
- Empirical formula: C₂₉H₂₅N₃O₇
- Molar mass: 527,52 g/mol
- Storage temperature: -20 °C
- Product type: Amine-reactive fluorescent dye
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about 5-Carboxytetramethylrhodamine succinimid, yl ester, 50 mg
What is this article?
5-Carboxytetramethylrhodamine succinimid, yl ester, 50 mg is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Fluorescent dyes & labels, inside Dyes, stains & indicators, in Lab chemicals.
A fluorescent dye absorbs at one wavelength and emits at a longer one. As a label it is attached to a protein, a nucleic acid or a cell. Excitation and emission maxima, and the reactive group if it must conjugate, are the specification. The colour name does not tell you the wavelengths.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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