Description
EC 4/3 NUCLEOSIL 300-5 C18 is an HPLC guard column designed to protect C18 analytical main columns in liquid chromatography systems. The cartridge measures 4 mm in length with a 3 mm internal diameter and is packed with NUCLEOSIL 300-5 C18 stationary phase. Its wide-pore octadecyl chemistry is well suited as a precolumn stage for methods that benefit from C18 selectivity, trapping debris and strongly retained contaminants before they compromise the main bed. Each unit is supplied as a pack of 3 pieces for main columns with 3, 4 or 4,6 mm internal diameter. Installation requires the Column Protection System guard column holder (REF 718966). This EC 4/3 guard format from manufacturer Macherey-Nagel supports reliable reversed-phase HPLC operation in research and quality-control environments. Regular guard replacement helps maintain retention stability, reduce pressure rise, and lower the frequency of full analytical column exchange during continuous sample analysis.
This article is part of HPLC columns, in HPLC columns & accessories.
Key features
- Pack size: 3 pc(s)
- Product type: HPLC guard column
- Stationary phase: NUCLEOSIL 300-5 C18
- Length: 4 mm
- Internal diameter: 3 mm
- EAN: 4046681081330
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Questions about EC 4/3 NUCLEOSIL 300-5 C18
What is this article?
EC 4/3 NUCLEOSIL 300-5 C18 is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
Which category should I open next?
The category is HPLC columns & accessories. The sub-category is HPLC columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

