Description
Fast Oxidation reagent, with ABI(TM) and PolyGen® synthesizer, for DNA synthesis, Density (D) 0,8 g/cm³, Flash point (flp) -21 °C, Boiling point (bp) 65 °C, Storage temp. +15 to +25 °C, UN-Nr. 1993
0,02 M Iode
The phosphite triester linkage formed during oligonucleotide synthesis is of limited stability and has to stabilised. Treatment of the support-bound material with iodine and water in the presence of a weak base oxidizes the phosphite triester into a tetracoordinated phosphate triester, a protected precursor of the naturally occurring phosphate diester internucleosidic linkage.