Description
HEPES Buffer 1M is a ready-to-use zwitterionic organic buffer solution designed to maintain physiological pH in cell culture media and related biochemical systems. The liquid formulation offers high solubility and is particularly recommended when cultures are sensitive to pH drift, extending buffering capacity within the range 7,2 to 7,6 independently of CO2 levels. Key attributes include membrane impermeability, limited interference with biochemical reactions, very low visible and ultraviolet light absorbance, and chemical as well as enzymatic stability. The pKa of HEPES is temperature-dependent: 7,48 at 25 °C and 7,31 at 37 °C. This convenient 1 M solution from manufacturer Sartorius is well suited to biochemistry, cell culture and molecular biology workflows where stable pH control is essential. Easy preparation and handling support routine laboratory use. The buffer helps stabilise media during open handling or in incubators with fluctuating CO2, contributing to reproducible experimental conditions without complex on-site formulation steps.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 1 pc
- Storage temperature: Room temperature
- Concentration: 1 M
- Form: Liquid solution
- Buffer type: Zwitterionic HEPES
- Working pH range: 7,2–7,6
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Questions about HEPES Buffer Solution 1.0 molar
What is this article?
HEPES Buffer Solution 1.0 molar is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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