Description
HPLC column NUCLEODUR®, 100-3 C8 ec, 250x4 mm
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Standard RP phase for daily routine analysis and up-scaling for preparative HPLC. This octyl modification is based on high purity NUCLEODUR® silica gel. C8 has a lower retention of hydrophobic compounds compared to C18 (octadecyl silane, ODS).
| Particle size | 3 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 250 x 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | C8 ec |
| Surface chemistry | Medium density monomeric octyl (C8, MOS) modification |
| USP listing | L7 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | hydrophobic (van der Waals interactions), some residual silanol interactions |
| Pressure stability | 600 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Routine analyses Aldehydes Basic, neutral or acidic drugs Derivatised amino acids Fat-soluble vitamins Ketones Pesticides |
| Similar phases | NUCLEOSIL® C8 NUCLEOSIL® C8 ec Spherisorb® C8 Symmetry® C8 Hypersil™ MOS Kromasil® C8 LiChrospher® RP8 |
| Application Database (Macherey-Nagel) | HPLC SPE |
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Questions about HPLC column NUCLEODUR®, 100-3 C8 ec, 250x4 mm, 1 unit(s)
What is this article?
HPLC column NUCLEODUR®, 100-3 C8 ec, 250x4 mm, 1 unit(s) is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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