Description
Ribonuclease A, 90 U/mg (Kunitz), BioScience-Grade, free from salt, Molar mass (M) ~13 700 g/mol, Storage temp. -20 °C, CAS No. 9001-99-4, EC No. 232-646-6
Extracted from bovine pancreas for RNA separation, especially when isolating RNA-free DNA. The enzyme cleaves RNA and produces 3' terminal nucleoside phosphates.This quality product for molecular biology is free from salt, without proteases and is chromatographically homogeneous. Unit definition acc. to Kunitz.
Directions for use
Working solution: 2-10 µg/ml in 10 mM Tris (pH 7,5), 15 mM NaCl.Note: RNase precipitates when concentrated solutions are heated/cooked at pH ≥7,0. Store solution in aliquots at -20 °C
Technical Information
| Instructions for use, addition | The DNase content of the RNase powder ist below detection limit. However, we nevertheless recommend pretreatment of the ribonuclease prior to using it for DNA isolation. DNase free RNase ist prepared by dissolution of the RNase powder in water, followed by cooking of the solubilised ribonuclease aliquots for 15 min. Let cool to room temperature on bench top. |