Description
Ribonuclease A, 90 U/mg (Kunitz), BioScience-Grade, free from salt, Molar mass (M) ~13 700 g/mol, Storage temp. -20 °C, CAS No. 9001-99-4, EC No. 232-646-6
This article belongs on Enzymes & substrates, in Biochemicals & reagents.
Extracted from bovine pancreas for RNA separation, especially when isolating RNA-free DNA. The enzyme cleaves RNA and produces 3' terminal nucleoside phosphates.This quality product for molecular biology is free from salt, without proteases and is chromatographically homogeneous. Unit definition acc. to Kunitz.
Working solution: 2-10 µg/ml in 10 mM Tris (pH 7,5), 15 mM NaCl.Note: RNase precipitates when concentrated solutions are heated/cooked at pH ≥7,0. Store solution in aliquots at -20 °C
| Instructions for use, addition | The DNase content of the RNase powder ist below detection limit. However, we nevertheless recommend pretreatment of the ribonuclease prior to using it for DNA isolation. DNase free RNase ist prepared by dissolution of the RNase powder in water, followed by cooking of the solubilised ribonuclease aliquots for 15 min. Let cool to room temperature on bench top. |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
Recently viewed
You may also like
Browse related categories
Category
Sub-category
Catalogue
Brand
Questions about Ribonuclease A, 90 U/mg (Kunitz), BioScience-Grade, free from salt, 100 mg
What is this article?
Ribonuclease A, 90 U/mg (Kunitz), BioScience-Grade, free from salt, 100 mg is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Life Science & Microbiology.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
Which category should I open next?
The category is Biochemicals & reagents. The sub-category is Enzymes & substrates. The catalogue is Life Science & Microbiology.
Shelves beside this one are Amino acids & peptides, Carbohydrates & sugars, Coenzymes, nucleotides & vitamins, Biochemicals (other), Antibiotics & antimycotics, Lipids, steroids & fatty acids and Detergents & surfactants.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.