Description
RIPA Buffer, 1x conc., ready-to-use,, for protein extraction, Density (D) 1,052 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +2 to +8 °C
The radioimmunoprecipitation buffer, also known as RIPA buffer, is one of the most reliable cell lysis buffers for fast and efficient lysis of mammalian cells from adhesion or suspension cultures.
It enables the extraction and solubilization of proteins from the membrane, the cell nucleus and the cytoplasm.
All the advantages at a glance:
- Extraction of proteins from numerous cell types
- Efficient cell lysis and solubilization of proteins
- Protein degradation is prevented
- No interference with most antibodies and protein antigens
- Minimizes non-specific protein binding interactions
- Compatible with many applications, including reporter assays, protein assays, immunoassays and protein purifications
Not a medical device / Not an IVD product
Technical Information
| Application | 1 ml RIPA buffer is sufficient for the lysis of 0,5 to 5 x 107cells (75 cm2 cell layer). |
| Instructions for use, addition | Protease and phosphatase inhibitors may be added to the lysis buffer as needed. |
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
H319
Causes serious eye irritation
Precautionary Statements (P-Phrases)
P280
Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.