Description
ROTI®Blot 1, discontinuous transfer is a ready buffer system for semi-dry blotting of proteins onto membranes in Western blot workflows. Supplied as a 1 kit glass pack, the formulation is provided as a 10× concentrate for electrophoresis-related transfer steps and supports discontinuous buffer arrangements used in semi-dry protein transfer. Density is 1 g/cm³. This kit from manufacturer Carl ROTH is intended for laboratories performing transfer of proteins to membranes within blotting, Western blot and ELISA-related applications. Discontinuous transfer buffers help establish defined ionic conditions across the gel–membrane stack, aiding efficient migration of polypeptides during semi-dry blotting. The concentrate format allows preparation of working solutions according to standard semi-dry protocols and routine bench practice. ROTI®Blot 1 fits application areas focused on blotting and protein transfer, where reproducible buffer composition is essential for consistent membrane binding ahead of immunodetection or staining. Use is aligned with electrophoresis transfer steps rather than continuous single-buffer systems.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 kit glass
- Density: 1 g/cm³
- Storage temperature: +15 to +25 °C
- Product type: buffer kit for semi-dry blotting
- Transfer mode: discontinuous
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
Recently viewed
You may also like
Questions about ROTI®Blot 1, discontinuous transfer, buffer for semi dry blotting,, 1 kit, glass
What is this article?
ROTI®Blot 1, discontinuous transfer, buffer for semi dry blotting,, 1 kit, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.