Description
ROTI®Blot A, anode buffer to ROTI®-Blot 1,, 10x conc. for electrophoresis, Density (D) 1,035 g/cm³, Storage temp. +15 to +25 °C
Discontinuous buffer system for optimum transfer in the semi-dry blotter.
- Optimised for semi dry blotting
- Superior blotting results for peptides and proteins of every size
- Special formulation with reduced acid formation - for optimal electrode protection
In the semi-dry blotting system, both a continuous buffer system (identical buffer at anode and cathode) and a discontinuous buffer system (different buffer at anode and cathode) can be used. The transfer performance of discontinuous systems is, however, generally higher, since the two buffers are designed separately according to the needs of the two electrodes. The discontinuous buffer system ROTI®Blot 1 consists of two different buffers, the anode buffer A (pH 7.8 ±0.1) and the cathode buffer K (pH 8.5 ±0.1). ROTI®Blot 1 shows a much better transfer efficiency than a tris-glycine buffer and can be used for proteins and peptides of any length.
The blot stack is built up by soaking the upper blotting papers with cathode buffer and the lower blotting papers with anode buffer. With slightly longer transfer times, proteins >100 kDa can also be completely transferred with ROTI®Blot 1.A detailed instruction manual is enclosed with the product.
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Blot A, anode buffer to ROTI®-Blot 1,, 1 l, glass
What is this article?
ROTI®Blot A, anode buffer to ROTI®-Blot 1,, 1 l, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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