Description
ROTI®Cell DMEM : F12 is a sterile, ready-to-use cell culture medium formulated with glutamine, pyruvate and HEPES under the CELLPURE® quality designation. It is supplied in a 500 ml plastic pack for direct use in cell culture workflows that require a DMEM/F12 basal medium with these supplements already included. This medium from manufacturer Carl ROTH is intended for laboratories performing routine and specialised culture of mammalian cells where a balanced DMEM : F12 blend supports growth and maintenance under controlled conditions. The ready-to-use format reduces preparation steps at the bench, while sterility supports aseptic handling in incubators and laminar-flow environments. Density is approximately 1 g/cm³ and the boiling point is about 100 °C, consistent with aqueous culture media. Plastic packaging suits standard cold-room and refrigerator logistics for media stocks. Researchers can apply ROTI®Cell DMEM : F12 in applications spanning general cell maintenance, expansion, and experimental setups that specify DMEM/F12 with glutamine, pyruvate and HEPES without further on-site formulation.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Glucose: 3.151 g/l
- L-Glutamine: complies
- HEPES: 15 mM
- pH value: 7.0-7.6
- Osmolality: 280-320 mOsm/kg
- Endotoxins: ≤1 EU/ml
- Sterility: complies
- Pack size: 500 ml plastic
- Storage temperature: +2 to +8 °C
- Product line: ROTI®Cell CELLPURE®
- Medium: DMEM : F12
- Formulation: With glutamine, pyruvate and HEPES
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTI®Cell DMEM , F12, sterile, with glutamine, pyruvate and, 500 ml, plastic
What is this article?
ROTI®Cell DMEM , F12, sterile, with glutamine, pyruvate and, 500 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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