Description
ROTI®CELL PBS/EDTA is a sterile phosphate-buffered saline containing 0,046 % EDTA and prepared without calcium or magnesium, intended for cell biology and cell dissociation work. Offered as CELLPURE® grade material, it shows a density of about 1 g/cm³ and a boiling point near 100 °C, making it a practical ready-to-use buffer for routine culture handling. The 500 ml plastic bottle format supports convenient bench use when gentle detachment of adherent cells is required and divalent cations must be avoided. This solution from manufacturer Carl ROTH fits life-science workflows that rely on controlled EDTA-assisted cell release under aseptic conditions. Its balanced PBS base helps maintain physiological ionic strength while the EDTA component chelates residual divalent ions that can interfere with dissociation steps. Laboratories performing passaging, harvest, or related cell-handling protocols can integrate the sterile pack directly into standard sterile technique without further formulation. Clear labelling of composition and pack size aids traceability in regulated or documented cell-culture environments.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- EDTA: 0.046 %
- pH value: 7.4-7.6
- Osmolality: 300-340 mOsm/kg
- Endotoxins: ≤1.0 EU/ml
- Sterility: complies
- Pack size: 500 ml plastic
- Density: ~1 g/cm³
- Product type: Sterile PBS/EDTA solution
- EDTA content: 0,046 %
- Composition: Without Ca/Mg
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTI®CELL PBS/EDTA, sterile, 0,046 % EDTA, w/o Ca/Mg, 500 ml, plastic
What is this article?
ROTI®CELL PBS/EDTA, sterile, 0,046 % EDTA, w/o Ca/Mg, 500 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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