Description
ROTI®CELL RPMI-1640 is a sterile, ready-to-use cell culture medium formulated with glutamine and HEPES for stable pH control outside CO2-intensive conditions. Offered under the CELLPURE® line, it is supplied in a 500 ml plastic bottle for immediate use at the laminar-flow cabinet. Density is about 1 g/cm³ and the boiling point is approximately 100 °C, in line with standard aqueous media. HEPES buffering complements the classic RPMI-1640 base, supporting cultures that experience temporary ambient handling or reduced incubator CO2 levels, while glutamine provides an essential nitrogen source for many mammalian lines. The medium from manufacturer Carl ROTH suits immunology, hybridoma, and general cell-biology workflows that specify RPMI with both glutamine and HEPES. Pre-sterilised liquid format removes reconstitution steps and reduces preparation variability between batches. This 500 ml pack size is practical for regular medium exchange in flasks and plates, giving cell-culture laboratories a convenient, fully supplemented RPMI-1640 option ready for everyday use.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- L-Glutamine: complies
- HEPES: 25 mM
- pH value: 7.0-7.6
- Osmolality: 280-310 mOsm/kg
- Endotoxins: ≤1,0 EU/ml
- Sterility: complies
- Pack size: 500 ml plastic
- Density: ~1 g/cm³
- Product line: ROTI®CELL CELLPURE®
- Medium: RPMI-1640
- Formulation: Sterile, with glutamine, with HEPES
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTI®CELL RPMI-1640, sterile, with glutamine, with HEPES, 500 ml, plastic
What is this article?
ROTI®CELL RPMI-1640, sterile, with glutamine, with HEPES, 500 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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