Description
ROTI®fair TG-Western, for 1000 ml / pouch, for electrophoresis, Density (D) 1,19 g/cm³
Exactly pre-weighed pouches for convenient and rapid preparation of TG-Western solution.
SDS-free running buffer for native protein gel electrophoresis. Optimum base for preparation of Western blot transfer buffer by addition of 5-20 % methanol (Blotting-Grade, Art. No. 0082).
Prepared solution may be sterilized by filtration (0,22 µm). For long-term storage, buffer solution should be kept at room temperature.
As ready-to-use mixtures, ROTI®Fair products reduce the user’s exposure to health-damaging substances used for preparation or pH adjustment. The production of ROTI®Fair tablets and sachets is carefully controlled in large quantities and can be made energy efficient. ROTI®Fair products reduce the total amount of waste produced and improve the footprint in the climate balance.
| Batch quantity | 1000 ml/pouch |
| End concentration | 1x |
| pH value | 8,3 ±0,05 |
| Buffer/solution | Tris-glycine buffer |
| Use | Buffer for protein electrophoresis and transfer |
| ROTH for Future Score - sustainability criteria | • Transport You can find further information on our website - Sustainability criteria at Carl ROTH. |
| ROTH for Future | yes |
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Protein biology & Western blot
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Protein extraction, purification & markers
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Questions about ROTI®fair TG-Western, for 1000 ml / pouch, 10 unit(s), box
What is this article?
ROTI®fair TG-Western, for 1000 ml / pouch, 10 unit(s), box is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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