Description
ROTI®Lumin, 10x conc., fluorecent-free, for protein and cytoimmunochemistry, Storage temp. +4 °C
- Sensitive and reliable
- For all standard membrane types
- Low background
- No cumbersome preparation of peroxidase buffers
- Suitable for multiple stripping and hybridisation
ROTI®Lumin is a kit for chemoluminescent detection of peroxidase-linked reporters (e.g. HRP-antibodies), as can be found in Western-Blot assays. In comparison to chromogen substrates, ROTI®Lumin displays increased sensitivity and reduced background reaction.
ROTI®Lumin is transformed by the HRP into an excited dianion in the presence of hydrogen peroxide. When the dianion is reversed back to its normal state, light is emitted. The light emission of ROTI®Lumin reaches its maximum within 5 minutes and remains at the maximum level for approx. 1-2 hours.
ROTI®Lumin consists of two solutions, which should be mixed at a 1:1 ratio before use. It can be used with nitrocellulose, nylon and PVDF membranes. Optimal results can be achieved with Immobilon-P® PVDF or ROTI®PVDF. Can be used for Western-Blotting, ELISA, Southern- Blotting, Dot-Blotting and colony hybridisation. Membranes may be stripped from bound probes using ROTI®Free stripping buffer, and may thus undergo multiple reprobing.
120 ml (25 ml) ROTI®Lumin 1 (Art. No. P079) and 120 ml (25 ml) ROTI®Lumin 2 (Art. No. P080).Contents of this Kit may not be bought separately.
| Application | Western, in situ, Histochemistry |
| Enzyme | Horseradish Peroxidase |
| Detection | chemoluminescent |
| Signal product | Precipitate (Blot) |
| Use | Western |