Description
ROTI®Nylon 0.2 transfer membrane is a neutrally charged nylon membrane supplied as a roll for nucleic acid blotting and related transfer workflows. The membrane has a pore size of 0,2 µm and is delivered in roll format with dimensions L 300 × W 30 cm, allowing sheets to be cut to the required blot size. Neutral charge supports transfer of nucleic acids onto the membrane surface in Southern and Northern blot applications and comparable laboratory transfer steps. Offered as one roll from manufacturer Carl ROTH, the product is intended for laboratories that prefer continuous membrane stock rather than pre-cut sheets. The roll format with a length of 300 cm and a width of 30 cm provides flexible cutting for different gel and apparatus formats while keeping a defined 0,2 µm pore size. Unit quantity is one roll, with a stated weight of 0,314 kg. ROTI®Nylon 0.2 in this roll presentation is positioned for transfer of nucleic acids to membranes within blotting workflows, giving a practical bulk format for routine and method-development use in molecular biology laboratories.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 roll
- Product type: Nylon transfer membrane, roll
- Brand grade: ROTI®Nylon 0.2
- Pore size: 0,2 µm
- Charge: Neutral charged
- Dimensions: L 300 × W 30 cm
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Questions about ROTI®Nylon 0.2, Transfermembran, Rolle, neutral charged, roll, L 300 x W 30 cm
What is this article?
ROTI®Nylon 0.2, Transfermembran, Rolle, neutral charged, roll, L 300 x W 30 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.