Description
ROTI®ProBlot PVDF Blotting Sandwich is a polyvinylidene fluoride membrane sandwich for nucleic-acid transfer in Southern and Northern blotting. With a pore size of 0,2 µm and an 85 x 135 mm sheet format, it fits larger gel systems commonly used for DNA and RNA analysis. The pack contains 20 pc(s), supporting parallel transfers and routine laboratory throughput. PVDF provides a robust medium for immobilisation of nucleic acids during electrophoretic transfer and downstream hybridisation or detection steps. This blotting sandwich from manufacturer Carl ROTH is arranged for straightforward incorporation into transfer stacks, reducing preparation time at the bench. Approximate pack weight is 0,150 kg. Apply within standard Southern and Northern workflows for transfer of nucleic acids to membranes whenever the 85 x 135 mm dimensions and 0,2 µm pore size align with the chosen electrophoresis and blotting apparatus.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Membrane material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,2 µm
- Dimensions: 85 x 135 mm
- Format: Blotting sandwich
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Questions about Blotting-Sandwich ROTI® ProBlot PVDF 0.2
What is this article?
Blotting-Sandwich ROTI® ProBlot PVDF 0.2 is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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