Description
ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, for DNA sequencing, Density (D) 1 g/cm³, Storage temp. +4 to +15 °C
- For preparation of 4-20 % sequencing gels
- Most simple handling of three optimised ready-to-use solutions
- No tedious dissolving of urea
- Prevents acrylamide dusts
- Reproducible, superior quality of gels
- In tried-and-tested ROTIPHORESE® quality
Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®Sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %.
Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).
- 1 l ROTIPHORESE®sequencing gel concentrate (Art. No. 3043.1) - ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1 with 50 % urea (8,3 M).
- 1 l ROTIPHORESE®sequencing gel diluent (Art. No. 3047.1) - 50 % (g/v; 8,3 M) solution of urea in water.
- 250 ml ROTIPHORESE®sequencing gel buffer concentrate (Art. No. 3050.1) - 10x TBE with 50 % urea (8,3 M).
- Detailed instructions for use are enclosed with the product.
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Questions about ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kit
What is this article?
ROTIPHORESE® DNA sequencing system, for 50 sequencing gels 30 x 40 cm, 1 kit is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in PCR, qPCR & nucleic acid analysis reagents, inside Molecular biology, in Life Science & Microbiology.
PCR and qPCR reagents are the polymerase, the nucleotides, the buffer and often a ready master mix, plus the dyes a real-time instrument reads. Hot-start enzymes and proofreading enzymes are different tools. A master mix already contains nucleotides. Adding them again is a failed reaction.
Molecular biology here is the handling of DNA and RNA. Extraction kits break the cell and then separate the nucleic acid from protein and from the other nucleic acid. PCR and qPCR reagents copy a chosen sequence so it can be seen. The enzymes and the nucleotides are what the copy depends on. Nuclease contamination destroys the sample before the kit can save it. Sequences are on NCBI Nucleotide. Papers are on PubMed.
Which category should I open next?
The category is Molecular biology. The sub-category is PCR, qPCR & nucleic acid analysis reagents. The catalogue is Life Science & Microbiology.
Shelves beside this one are DNA & RNA extraction kits and Molecular biology reagents (other).
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