Description
Toluidine blue O (C.I. 52040) is a cationic thiazine dye supplied for microscopy, with CAS number 92-31-9, empirical formula C₁₅H₁₆ClN₃S and molar mass 305,83 g/mol. This well-established biological stain is used in histological and cytological workflows where metachromatic colouration of tissue components, mucopolysaccharides and nucleic acids supports clear morphological assessment under the light microscope. The dye is With EG number 202-146-2, the material is clearly identified for laboratory documentation and inventory control. Prepared for microscopy applications, Toluidine blue O from manufacturer Carl ROTH fits routine biological staining benches in pathology, cell biology and educational laboratories. Its defined chemical identity and convenient pack size support reproducible preparation of working stains for sectioned specimens and smear preparations.
This article is part of Microscopy stains & staining solutions, in Dyes, stains & indicators.
Key features
- Appearance: dark green to black powder
- Dye content: ≥65 %
- Absorption max. (in H2O): 615-635 nm
- CAS number: 92-31-9
- Pack sizes: 5 g, 10 g, 25 g, 100 g
- EG nr.: 202-146-2
- Empirical formula: C₁₅H₁₆ClN₃S
- Molar mass: 305,83 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about Toluidine blue O (C.I. 52040), for microscopy, 5 g, glass
What is this article?
Toluidine blue O (C.I. 52040), for microscopy, 5 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Microscopy stains & staining solutions, inside Dyes, stains & indicators, in Lab chemicals.
A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
Which category should I open next?
The category is Dyes, stains & indicators. The sub-category is Microscopy stains & staining solutions. The catalogue is Lab chemicals.
Shelves beside this one are Fluorescent dyes & labels and Dyes & pigments.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.