Description
TRIS (tris(hydroxymethyl)aminomethane), PUFFERAN® Ultra quality, is a high-purity buffer base with the empirical formula C₄H₁₁NO₃ and CAS number 77-86-1. Specified at a minimum purity of 99,9 %, it has a molar mass of 121,14 g/mol, a density of 1,32 g/cm³ and a boiling point of 288 °C. This is intended for preparing reliable buffer systems in biochemistry, molecular biology and analytical laboratories. TRIS is a standard component of electrophoresis, chromatography and cell-culture media formulations where stable pH control is essential. Offered by manufacturer Carl ROTH under the PUFFERAN® Ultra quality designation, the material supports demanding workflows that require a well-characterised amino-alcohol buffer salt. quantity is practical for routine buffer stock preparation, method validation and larger experimental series. Laboratories seeking a consistent TRIS grade for biochemical buffering, sample preparation and related life-science applications can rely on this defined Ultra quality material for day-to-day use.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Assay (dry substance): ≥99,9 %
- In H2O insoluble matter: ≤0,005 %
- pH-value (5 % solution): 10,0-11,5
- Melting point: 168-172 °C
- Water: ≤0,5 %
- Heavy metals (as Pb): ≤0,0001 %
- CAS number: 77-86-1
- Pack sizes: 500 g, 5 kg, 1 kg, 10 kg, 2.5 kg
- EG nr.: 201-064-4
- Purity: min. 99,9 %, Ultra quality
- Empirical formula: C₄H₁₁NO₃
- Molar mass: 121,14 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about TRIS, PUFFERAN®, min. 99,9 %, Ultra quality, 500 g, plastic
What is this article?
TRIS, PUFFERAN®, min. 99,9 %, Ultra quality, 500 g, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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