Description
TRIS-Acetate-EDTA buffer pH 8,5 (50×) GEN is a concentrated molecular-biology buffer concentrate supplied in a 1 L pack for laboratory nucleic-acid workflows. The 50× stock is formulated at pH 8,5 and is intended to be diluted to working strength before use in electrophoresis and related buffering applications where TRIS-acetate-EDTA systems are specified. As a ready concentrate, it simplifies preparation of running or sample buffers and helps maintain consistent ionic conditions once diluted according to protocol. This buffer from manufacturer Labbox belongs to the LABKEM product line and is positioned for routine molecular-biology bench work. The net weight is 1,610 kg for the filled pack. Typical use includes agarose-gel electrophoresis and other procedures that call for TAE-type buffering at the stated pH after appropriate dilution from the 50× concentrate. Handle and store according to laboratory good practice for buffer concentrates; dilute only as required for the intended method. The product is offered as one package unit for stock preparation in research and teaching laboratories.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 1 L
- Product type: TRIS-Acetate-EDTA buffer pH 8,5 (50×) GEN
- Concentration: 50× concentrate
- pH value: 8,5
- EAN: 8434868060012
- Technical Information Sheet:
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Questions about TRIS-Acetate-EDTA-Buffer pH 8.5 (50x) GEN, 1 L
What is this article?
TRIS-Acetate-EDTA-Buffer pH 8.5 (50x) GEN, 1 L is the article on this page. Luminix Health distributes Labbox for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
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The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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