Description
3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., Molar mass (M) 240,35 g/mol, Storage temp. +4 °C, CAS No. 54827-17-7, EC No. 259-364-6, Empirical formula C16H20N2
Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.
Dissolves easily in acetone, chloroform and ethanol.
Directions for use
Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H2O2 ad 0.01 %. Always prepare freshly!Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H2SO4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).Protect from light.
Technical Information
| Enzyme | Horseradish Peroxidase |
| Detection | Colour (blue) |
| Signal product | soluble (ELISA) |