Beschrijving
PIPES is een zeer zuiver zwitterionisch bufferreagens, geleverd als PUFFERAN®-kwaliteit met een minimale gehaltebepaling van 99 %, veel gebruikt in de eiwitbiologie en verwante life-science-toepassingen. Het heeft de empirische formule C₈H₁₈N₂O₆S₂, een molaire massa van 302.37 g/mol, CAS-nummer 5625-37-6 en EG-nummer 227-057-6. is gedimensioneerd voor het bereiden van stock- en werkbuffers in workflows voor eiwitisolatie, zuivering en karakterisering. Dit bufferzout van fabrikant Carl ROTH biedt betrouwbare pH-regeling in het bijna-fysiologische bereik en is een standaardkeuze waar Good’s buffers de voorkeur hebben. Een hoge minimale zuiverheid ondersteunt een reproduceerbare buffersamenstelling voor gevoelig biomoleculair werk. Laboratoria kunnen de vrije zuurvorm oplossen en titreren tot de vereiste werk-pH voor chromatografie-, extractie- of assaybuffers. Gedocumenteerde CAS-, EG- en molaire-massagegevens vereenvoudigen specificatie en traceerbaarheid in eiwitzuivering en reagentia-voor-isolatieprotocollen.
Dit artikel maakt deel uit van Biologische buffers (Tris, HEPES, MOPS), in Buffers & pH-oplossingen.
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Questions about PIPES, PUFFERAN®, min. 99 %, 25 g, kunststof
What is this article?
PIPES, PUFFERAN®, min. 99 %, 25 g, kunststof is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biologische buffers (Tris, HEPES, MOPS), inside Buffers & pH-oplossingen, in Chemicaliën.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH-oplossingen. The sub-category is Biologische buffers (Tris, HEPES, MOPS). The catalogue is Chemicaliën.
Shelves beside this one are pH-buffers & geleidbaarheidsstandaarden and Buffers (overig).
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The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
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Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

