Beschrijving
SDS-PAGE sample buffer concentrated (reducing) is a pharmacopoeia-quality laboratory reagent for preparing protein samples under reducing conditions for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). It is specified as Reag. Ph. Eur. in accordance with European Pharmacopoeia Chapter 4.1.1 under reagent number 1122100, and is grouped with reagents and volumetric standard solutions of pharmacopoeial quality. The concentrated reducing buffer is supplied in a 500 ml pack. A reducing SDS-PAGE sample buffer is combined with protein solutions so that disulfide bonds can be reduced and polypeptides coated with detergent before loading onto polyacrylamide gels; the concentrated format lets the laboratory set the working ratio. This reagent from manufacturer Carl ROTH belongs to the Ph. Eur. Reagents 4.1.1 assortment used in analytical and biochemical laboratories that require pharmacopoeia-referenced chemicals. The 500 ml unit, corresponding to a net weight of 0,700 kg, is suited to routine sample preparation for reducing SDS-PAGE.
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