Description
ROTI®ProBind PVDF Transfer Membrane is a polyvinylidene fluoride transfer membrane for laboratory blotting, including Southern and Northern blot work and transfer of nucleic acids to membranes. It offers a pore size of 0,45 µm on a continuous roll measuring 300 × 30 cm, so sheets can be cut to match common gel and cassette formats. The pack is one roll with a weight of 0,680 kg, suited to molecular biology benches that need a ready PVDF stock for immobilisation steps. This ROTI®ProBind membrane from manufacturer Carl ROTH supports routine nucleic acid transfer workflows where mechanical handling and consistent pore geometry matter during blotting. PVDF is a practical choice when membranes must withstand washing, probing and detection sequences after transfer. Cutting from the roll helps match different blot sizes and reduces leftover scrap in multi-user labs. Use it in standard Southern and Northern protocols and related membrane-based nucleic acid transfer applications that specify 0,45 µm PVDF.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 roll
- Product type: PVDF transfer membrane
- Material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,45 µm
- Dimensions: 300 × 30 cm
- Weight: 0,680 kg
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Questions about Transfer membrane ROTI® ProBind PVDF 0.45, 300 × 30 cm
What is this article?
Transfer membrane ROTI® ProBind PVDF 0.45, 300 × 30 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.