Roti®Nylon Plus Reprobe, positively charged, 0.45 µm, 20 × 20 cm, 5 sheets, Carl Roth, art. 261H.1

Blotting membranes & papers

Here you will find everything your laboratory needs for blotting membranes and papers, such as: transfer membrane, papers, blotting papers grade, blotting paper and pore size. This range is for biotech, hospitals, R&D, teaching and (quality) control. This collection holds 143 articles. The catalogue holds more than 150.000 articles. In-stock articles ship in two to five business days.

143 products

Showing 1 - 24 of 143 products

Showing 1 - 24 of 143 products
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ROTI®PVDF 0.2, pore size 0,2 µm, 7 × 8,4 cm, Carl Roth, art. 23HH.1Carl Roth
Carl Roth ROTI®PVDF 0.2, pore size 0.2 µm, 10 sheet(s), 7 x 8,4 cm , 10 sheet(s)
From €106,70
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ROTI®PVDF 0.2, pore size 0,2 µm, 20 x 20 cm, 5 sheets, Carl Roth, art. 23HC.1Carl Roth
Carl Roth ROTI®PVDF 0.2, pore size 0.2 µm, 5 sheet(s), 20 x 20 cm, 5 sheet(s)
From €147,06
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Amersham™ Protran® 0,45 µm NC membrane, roll 300 mm × 4 m, Cytiva, art. 4675.1Cytiva
Cytiva Amersham(TM) Protran® 0.45 µm NC, roll, 300 mm x 4m, 1 roll(s)
From €623,07
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ROTI®PVDF 0.2, pore size 0,2 µm, 9 x 10 cm, 10 sheets, Carl Roth, art. 23H9.1Carl Roth
Carl Roth ROTI®PVDF 0.2, pore size 0.2 µm, 10 sheet(s), 9 x 10 cm, 10 sheet(s)
From €136,98
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ROTI®PVDF 0.2, pore size 0,2 µm, roll 300 x 30 cm, Carl Roth, art. 1YYC.1Carl Roth
Carl Roth ROTI®PVDF 0.2, pore size 0.2 µm, Roll, 300 x 30 cm, PVDF Membrane, 1 roll(s)
From €427,64
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Immobilon-P PVDF membrane, pore size 0,45 µm, roll 375 x 30 cm, Merck Millipore, art. T831.1MERCK MILLIPORE
MERCK MILLIPORE Immobilon-P, pore size 0,45 µm, roll, 375 x 30 cm, PVDF-membrane, 1 roll(s)
From €542,79
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ROTI®NC, transfer membrane, sheets — 0,2 µm, Carl Roth, art. 204H.1Carl Roth
Carl Roth ROTI®NC, transfer membrane, sheets, Nitrocellulose, 10 x 10 cm, pore size 0.2 µm
From €111,83
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ROTI®NC, transfer membrane, roll — 0,2 µm, Carl Roth, art. 200L.1Carl Roth
Carl Roth Transfer membrane ROTI®NC 0.45, 300 × 20 cm
From €233,19
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ROTI®NC, transfer membrane, sheets — 0,45 µm, Carl Roth, art. 200P.1Carl Roth
Carl Roth ROTI®NC, transfer membrane, sheets, Nitrocellulose, 10 x 10 cm
From €91,31
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ROTI®NC, transfer membrane, sheets — 0,45 µm, Carl Roth, art. 200N.1Carl Roth
Carl Roth ROTI®NC, transfer membrane, sheets, nitrocellulose, 20 x 20 cm
From €106,43
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ROTI®NC 0.45, transfer membrane, roll — 0,45 µm, Carl Roth, art. 200K.1Carl Roth
Carl Roth ROTI®NC 0.45, transfer membrane, roll, Nitrocellulose, 30 x 300 cm, 1 roll(s)
From €365,72
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ROTI®NC, transfer membrane, roll — 0,2 µm, Carl Roth, art. 200E.1Carl Roth
Carl Roth Transfer membrane ROTI®NC 0.2, 300 × 20 cm
From €249,62
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qpore® Transfer membrane, PVDF, 0,22 µm, 300 x 3000 mm roll, neoLab, art. 60002neolab
neolab qpore® transfer membrane of PVDF, 0.22 µm, 300 x 3000 mm roll
€360,00
0,22 µm nitrocellulose blotting membrane, Sartorius, art. 1132741BLSartorius
Sartorius CN 0.22 µm, roll 300mm x 3m,Blotting
€356,00
qpore® Transfer membrane, CN, 0,45 µm, 300 x 3000 mm roll, neoLab, art. 60001neolab
neolab qpore® transfer membrane of CN, 0.45 µm, 300 x 3000 mm roll
€309,00
ROTI®NC, transfer membrane, sheets — 0,2 µm, Carl Roth, art. 200H.1Carl Roth
Carl Roth ROTI®NC, transfer membrane, sheets, nitrocellulose, 20 x 20 cm, pore size 0.2 µm
From €122,09
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qpore® Transfer membrane, PVDF, 0,45 µm, 300 x 3000 mm roll, neoLab, art. 60003neolab
neolab qpore® transfer membrane of PVDF, 0.45 µm, 300 x 3000 mm roll
€360,00
qpore® Transfer membrane, CN, 0,22 µm, 300 x 3000 mm roll, neoLab, art. 60000neolab
neolab qpore® transfer membrane of CN, 0.22 µm, 300 x 3000 mm roll
€309,00
ROTI®NC, Transfer membrane, roll — 0,2 µm, Carl Roth, art. 1YXN.1Carl Roth
Carl Roth ROTI®NC, Transfer membrane, roll, Nitrocellulose, 30 x 300 cm, 1 roll(s)
From €364,77
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0,45 µm nitrocellulose blotting membrane, Sartorius, art. 1130641BLSartorius
Sartorius CN 0.45 µm, Roll 300mm x 3m,Blotting
€356,00
Blotting paper, grade BF 4, 580 × 600 mm sheets, Sartorius, art. FT2521580600GSartorius
Sartorius Blotting papers/ Grade BF 4
€265,00
Blotting paper / Grade BF 4 / 580 × 580 mm sheets, Sartorius, art. FT2521580580GSartorius
Sartorius Blotting papers/ Grade BF 4
€331,00
Blotting paper Grade BF 4, 110 × 170 mm sheets, Sartorius, art. FT2521110170GSartorius
Sartorius Blotting papers/ Grade BF 4
€48,90
Blotting paper Grade BF 4, 150 × 150 mm sheets, Sartorius, art. FT2521150150GSartorius
Sartorius Blotting papers/ Grade BF 4
€54,20

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Questions about Blotting membranes & papers

Nitrocellulose or PVDF: which blotting membrane should I choose?

Nitrocellulose binds proteins well, gives low background and needs no activation. PVDF is mechanically stronger, withstands stripping and reprobing, and suits protein sequencing; wet it briefly in methanol before use. Nylon membranes are mainly used for DNA and RNA blots. For a standard Western blot, both nitrocellulose and PVDF work. Pore size matters too: smaller pores retain small proteins better. Related products are in protein biology and Western blot. For a quote or a second opinion, write to sales@luminixhealth.com.

0.2 or 0.45 µm pore size?

A 0.45 µm membrane suits most proteins in a routine Western blot. Choose 0.2 µm for small proteins and peptides, which can pass through a wider pore during transfer and get lost. Check the pore size and the protein range of a membrane on the product page and compare them with your target before ordering. Both versions are in blotting membranes and papers. Questions about a specific product? Contact sales@luminixhealth.com.

Which blotting paper (filter paper) do I need for wet or semi-dry blotting?

For semi-dry blotting, use thick, absorbent paper, because it holds the transfer buffer that the system needs. In a wet tank, thinner paper in the blotting sandwich is enough. Cut the sheets to the size of your gel, or choose pre-cut sheets to save time and get even contact. Tanks, blotters and power supplies are in electrophoresis systems and power supplies. Unsure which one fits? Ask sales@luminixhealth.com.

What do I need after transfer: blocking and incubation?

First block the membrane with a blocking agent, such as skimmed milk powder or BSA, so free binding sites are occupied and the antibody binds only to your target. Then incubate in a tray with the primary and secondary antibodies, with wash steps in between. Small trays save antibody solution. Follow the incubation times and dilutions given in your protocol. Keep the membrane wet throughout, as a dried membrane gives high background. Antibodies are in primary antibodies and secondary antibodies. Need advice for your set-up? Mail sales@luminixhealth.com.

Why Blotting membranes & papers?

A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.

Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.

This shelf belongs to Protein biology & Western blot, inside Microbiology lab supplies. Luminix Health distributes this range for laboratory use.

Which shelves sit beside this one?

The neighbouring shelves are Protein biology (other) and Protein extraction, purification & markers. The category above this shelf is Protein biology & Western blot.

How does ordering and shipping work?

Luminix Health distributes this range from the heart of Europe. We deliver across the EU in two to five days if in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. Send a quote request, contact the team, or read who we are. The full brand index is on shop by brand.

Which other categories should I open?

The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology. If the article is not on the shelf, request a quote.