Description
3,3'-Diaminobenzidine tetrahydrochloride, min. 98 %, p.a., Molar mass (M) 360,10 g/mol, Flash point (flp) 282,7 °C, Boiling point (bp) 481,7 °C, Storage temp. +4 °C, CAS No. 7411-49-6, EC No. 231-018-9, Empirical formula C12H14N4 · 4 HCl
Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage.
Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.
| Appearance | fast weißes bis braunes Pulver |
| Application | Western, in situ, Histochemistry |
| Enzyme | Horseradish Peroxidase |
| Detection | Colour (brown) |
| Signal product | Precipitate (Blot) |
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about 3,3'-Diaminobenzidine tetrahydrochloride, min. 98 %, p.a., 1 g, glass
What is this article?
3,3'-Diaminobenzidine tetrahydrochloride, min. 98 %, p.a., 1 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein biology (other), inside Protein biology & Western blot, in Life Science & Microbiology.
This shelf is the protein-biology article that is not a membrane and not an extraction kit: stains for gels, buffers, small tools of a protein bench. The method, a gel stain or a quantitation assay, still chooses the bottle.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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