Description
4-Nitrophenyl phosphate disodium salt, hexahydrate, min. 99 %, for biochemistry, Molar mass (M) 371,12 g/mol, Storage temp. -20 °C, CAS No. 4264-83-9, EC No. 224-246-5, Empirical formula C6H4NNa2O6P · 6 H2O
Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.
Directions for use
Working solution: 1 mg/ml in diethanolamine substrate buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5). For endpoint analysis the reaction may be stopped via addition of 2-3 M NaOH.
Technical Information
| Enzyme | Alkaline phosphatase |
| Detection | Colour (yellow) |
| Signal product | soluble (ELISA, activity assay) |