Description
anti-Desmoglein 2 mouse, monoclonal, 10G11, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
Antibody recognizes desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide reacting: desmoglein 2 (1069aa; calculated mol. wt. 116,760) Reactivity on cultured cell lines: many, including CaCo-2, HaCaT, MCF-7
Immunohistochemistry (IHC) - frozen: 1:100 (5 μg/ml, for better resolution preincubation (directly after fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, is recommended (Schäfer S. et al., 1996)) Western Blot (WB): assay dependent
| Application | IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | mouse rat |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 500 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | Q14126 (human) |
| Clone | 10G11 |
| Target | recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain) |