Primary antibodies

A monoclonal antibody recognises one epitope and comes from a single clone, so batches are highly consistent and specificity is high. That suits quantitative assays, diagnostics and long-running studies. The clone name is in the title, for example the mouse monoclonal anti-CDK4, clone DCS-156 or the mouse monoclonal anti-GST tag. A polyclonal antibody binds several epitopes on the same target, which often gives a stronger signal and tolerates small changes in the protein, useful for detection of low-abundance or denatured targets. Batch-to-batch variation is larger, though. Questions: sales@luminixhealth.com.

An isotype control is an antibody of the same species and isotype as your primary antibody, but without specificity for your target. Run it in parallel under the same conditions to see how much signal comes from non-specific binding, for instance to Fc receptors in flow cytometry or to tissue in immunohistochemistry. Match it exactly: a mouse IgG2a kappa primary needs a mouse IgG2a kappa isotype control, a mouse IgG1 primary a mouse IgG1 isotype control, and a rabbit primary a rabbit IgG isotype control. Use the same concentration as the primary.

A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.

An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.

This shelf belongs to Antibodies & ELISA kits, inside Microbiology lab supplies. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are ELISA kits, Recombinant proteins & cytokines and Secondary antibodies. The category above this shelf is Antibodies & ELISA kits.

Luminix Health distributes this range from the heart of Europe. We deliver across the EU in two to five days if in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. Send a quote request, contact the team, or read who we are. The full brand index is on shop by brand.

The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology. If the article is not on the shelf, request a quote.