Description
BIS-TRIS is a high-purity biological buffer substance supplied as PUFFERAN® grade with a minimum assay of 99 %. Identified by CAS number 6976-37-0 and empirical formula C₈H₁₉NO₅, it has a molar mass of 209,24 g/mol, a density of approximately 1,4 g/cm³ and a boiling point of 412,1 °C. The EG number is 230-237-7. This zwitterionic amino-alcohol buffer is widely used in molecular biology and life-science workflows where stable pH control is required for electrophoresis, protein work and nucleic-acid procedures. Offered from manufacturer Carl ROTH within the PUFFERAN® range, BIS-TRIS supports reproducible buffering performance in research and analytical settings. Its defined physicochemical profile allows accurate formulation of working buffers for molecular biological applications. The solid is suited to laboratories that need a reliable, well-characterised buffer component for day-to-day molecular biology reagent preparation and related experimental protocols.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Assay (titr.): ≥99 %
- Loss of drying (90 °C, 30 min): ≤1.0 %
- pH value (1 M, H2O): 9.0-11.0
- Solubility (1 M in H2O): clear, soluble
- Iron (Fe): ≤0,0003 %
- Heavy metals (as Pb): ≤0,0003 %
- 340 nm: ≤0,02
- 280 nm: ≤0,04
- CAS number: 6976-37-0
- Pack sizes: 50 g, 250 g, 500 g, 5 kg, 1 kg
- EG nr.: 230-237-7
- Purity: min. 99 %, PUFFERAN®
- Empirical formula: C₈H₁₉NO₅
- Molar mass: 209,24 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about BIS-TRIS, PUFFERAN®, min. 99 %, 50 g, plastic
What is this article?
BIS-TRIS, PUFFERAN®, min. 99 %, 50 g, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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