Description
Dulbecco's Phosphate Buffered Saline without Calcium and Magnesium is intended to provide a buffer system for maintaining cell culture media in the physiological pH range of 7,2–7,6. It generally serves as an isotonic saline solution or buffer for washing cells and tissues when a divalent cation-free matrix is preferred. The formulation is modified without calcium and without magnesium.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Available as 1 pc with a net weight of 0,100 kg and a gross weight of 0,140 kg, this DPBS preparation from manufacturer Sartorius is suited to routine rinse and wash steps in cell culture. Omitting calcium and magnesium supports protocols such as pre-dissociation washing or other handling steps where Ca²⁺ and Mg²⁺ could interfere. As an isotonic phosphate-buffered saline, it helps preserve osmotic balance during short-term processing of cells and tissue samples.
Laboratories choose this calcium- and magnesium-free Dulbecco's PBS when standard wash or buffer procedures specify the absence of these ions while still targeting the physiological pH window of 7,2–7,6. It is a practical option for everyday cell and tissue washing under defined, divalent cation-free conditions.
Key features
- Pack size: 1 pc
- Storage temperature: Room temperature
- Product type: Dulbecco's Phosphate Buffered Saline (DPBS)
- Modification: Without calcium and magnesium
- pH range: Physiological 7,2–7,6
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Questions about Dulbecco's PBS w/o CA & MG 100ML
What is this article?
Dulbecco's PBS w/o CA & MG 100ML is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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